Journal: Photonics

Article Title: Combined TPEF and SHG Imaging for the Microstructural Characterization of Different Wood Species Used in Artworks

doi: 10.3390/photonics9030170

Figure Lengend Snippet: Figure 2. NLO microscopy images of poplar shavings cut along the direction of the stem on the tangential plane (excitation wavelength 840 nm). Bordered pits are pointed out by light-blue ar- rowheads. Greyscale intensity images (a) 100 × 100 µm2 and (b) 200 × 200 µm2 are obtained by optically summing all excited nonlinear signals, thus showing both SHG and TPEF signals indis- tinctively. (c) TPEF/SHG merged image (200 × 200 µm2) enabling the distinction between TPEF (green-coded) and SHG (red-coded) signals, attributed to lignin and starch (pointed out by the red arrowheads), respectively.

Article Snippet: Photonics 2022, 9, x FOR PEER REVIEW 7 of 11 Figure 3. (a) Poplar greyscale NLO microscopy images (200 × 200 μm2) and (b) corresponding TPEF/SHG merged images acquired at different depths inside the wood slice (tangential section), highlighting the presence of SHG emitters.

Techniques: Microscopy, Starch

Journal: Photonics

Article Title: Combined TPEF and SHG Imaging for the Microstructural Characterization of Different Wood Species Used in Artworks

doi: 10.3390/photonics9030170

Figure Lengend Snippet: Figure 3. (a) Poplar greyscale NLO microscopy images (200 × 200 µm2) and (b) corresponding TPEF/SHG merged images acquired at different depths inside the wood slice (tangential section), highlighting the presence of SHG emitters. Depths of recording from the sample’s surface are indicated in the top right corner (81 µm scanning depth; 1 µm sampling step).

Article Snippet: Photonics 2022, 9, x FOR PEER REVIEW 7 of 11 Figure 3. (a) Poplar greyscale NLO microscopy images (200 × 200 μm2) and (b) corresponding TPEF/SHG merged images acquired at different depths inside the wood slice (tangential section), highlighting the presence of SHG emitters.

Techniques: Microscopy, Sampling

Journal: Photonics

Article Title: Combined TPEF and SHG Imaging for the Microstructural Characterization of Different Wood Species Used in Artworks

doi: 10.3390/photonics9030170

Figure Lengend Snippet: Figure 4. Beech NLO microscopy images (excitation wavelength 850 nm), showing the fibers (light- blue arrowheads) in the tangential section and the aggregates of parenchyma rays (yellow arrow- heads) in the transverse section. Images are obtained from the maximum-intensity projection over all acquired images in a stack (101 µm scanning depth; 1 µm sampling step), thus enhancing the visual- ization of the inner details. (a,c) TPEF and SHG signals generated from lignin and cellulose/starch are optically summed. (b,d) Merged TPEF/SHG image showing the morphology of rays and fibers, as well as the distribution of SHG and fluorescence emitters.

Article Snippet: Photonics 2022, 9, x FOR PEER REVIEW 7 of 11 Figure 3. (a) Poplar greyscale NLO microscopy images (200 × 200 μm2) and (b) corresponding TPEF/SHG merged images acquired at different depths inside the wood slice (tangential section), highlighting the presence of SHG emitters.

Techniques: Microscopy, Sampling, Generated, Starch

Journal: Photonics

Article Title: Combined TPEF and SHG Imaging for the Microstructural Characterization of Different Wood Species Used in Artworks

doi: 10.3390/photonics9030170

Figure Lengend Snippet: Figure 6. (a) Greyscale NLO microscopy image of chestnut and (b) orthogonal views of a merged TPEF/SHG image stack showing the presence of isolated aggregates of material strongly emitting SHG signals (red-coded) and TPEF signals (green-coded) generated by lignin.

Article Snippet: Photonics 2022, 9, x FOR PEER REVIEW 7 of 11 Figure 3. (a) Poplar greyscale NLO microscopy images (200 × 200 μm2) and (b) corresponding TPEF/SHG merged images acquired at different depths inside the wood slice (tangential section), highlighting the presence of SHG emitters.

Techniques: Microscopy, Isolation, Generated